Monkeypox testing

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Please be aware that pre-approval for monkeypoxvirus/orthopoxvirus testing at CDPHE is required per guidelines set by the laboratory response network.

 The CPT code for monkeypox is 87593.

Please request testing supplies using this form: Public Health Testing Supply Order Form 

  • The option for monkeypox test kits is found at the bottom of the second page of the form.

  • Test kits include swabs, conical tubes, and specimen collection bags.

The pre-approval and test ordering process for monkeypox is now available in LabOnline.  LabOnline is an online portal where you can order tests and view results from the state lab.

  1. The pre-approval screening questions are found at the beginning of the monkeypox test ordering process in LabOnline. Please complete these questions first to gain approval to collect a specimen from the patient.

  2. Testing approval may be dependent on whether the patient:

    • Has a rash, lesions, or bumps that resemble monkeypox.

    • Is a man who identifies as gay or bisexual or reports sexual or intimate contact with other men.

    • Is experiencing homelessness or lives in a congregate setting.

    • Had direct skin-to-skin contact with a person diagnosed with monkeypox or experiencing similar symptoms.

    • Had contact with a dead or live wild animal or exotic pet that is an African endemic species or used a product derived from such animals (e.g., game meat, creams, lotions, powders, etc.).

  3. If testing is indicated, collect a specimen from a maximum of two different lesions using dry swabs. Each lesion must be swabbed twice. Place each swab in its own transport tube.

  4.  A test order must be submitted for each lesion location for a patient. Insert the requisition form into the outer pocket of the specimen collection bag.

    • For example, if specimens are collected from the left leg and right arm of a patient, you will order a test for the specimens collected from the left leg, and then order another test for the specimens collected from the right arm. In the end, two requisition forms with two different chain of custody numbers (the number found under the bar code of the form) will be generated. 

  5.  When labeling the specimen, please list the following on the tube label:

    • Patient first and last name.

    • Date of birth.

    • Collection date AND time.

    • Lesion location.

  6.  Store the collected samples at refrigerated temperatures while waiting for pick-up.

Place a request to pick up monkeypox samples using the courier request form.

  • A courier request can be made 24-48 hours prior to pick-up.

  • If you have any courier related emergencies, please call (720) 507-5917.

Progress and results of a test can be viewed in LabOnline.

This video walks through the above steps.

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